17+ Ligation Calculator
Are you carrying out the ligation reaction under high salt conditions 100 mM NaCl. This tool will calculate the mass of insert required at several molar insertvector ratios in the range needed for typical ligation reactions.
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Then carefully enter the coefficients of each reactant and product in the.
. These links can help you calculate how much insert and backbone you need for different types of cloning methods such as restriction. Web LIGATION CALCULATOR Please provide the following information. Web Download the Ligation Calculator app for Android Android 23 Gingerbread Android 22 Froyo Total Reaction Mixµl Volume of the bufferµl Volume of the enzymeµl Vector.
Web This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches. Web How much 500bp insert DNA needs to be added to 100ng of 30kb vector in a ligation reaction for a desired vectorinsert ratio of 13. Web First click on the ligation calculator module.
Please enter the molar vector. 100ng vector 05kb insert. Vector amount in ng.
Purpose Key Facts Contents Vector size Vector amount. Vector size in bp. Web N 1 V 1 N 2 V 2 Substituting in the equation 20 V 1 10 V 2 or V 1 V 2 05 So we have to dilute the solution to half of its concentration.
Single base 1 nt TA overhangs. Insert size in bp. Options include conversion of mass to moles ligation amounts conversion of OD.
Web Ligation Calculator Determines the optimal insert amount for a cloning reaction based on vector and insert properties. Web The NEBioCalculator Ligation module can help you plan a successful ligation reaction as part of a restriction cloning workflow by optimizing the molar ratio between your vector. Enter 2 kb for your DNA insert length and 6 kb for your vector DNA length making sure that the units selected are correct.
Use this tool for your scientific calculations and conversions for DNA and RNA. For example in NEBuffer 31. Convert double strand DNA ds DNA to pmoles and ends.
Web Cloning ligation reaction calculators. Web In this molar ratio calculator first set Select calculation type to Calculate molar ratio. Ds DNA size in base pairs bp Quantity in micogram ug PART 2.
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